CHARACTERISATION OF THE MICROBIAL COMMUNITY IN INDOOR ENVIRONMENTS: A CHEMICAL-ANALYTICAL APPROACH
Original hosted in "MACE", contributed by Social Updater
Gas chromatography/ion trap-tandem mass spectrometry (GC/MS/MS) was used to determine 3-hydroxy fatty acids with 10-18 carbon atoms, branched-chain fatty acids with 15 and 17 carbon atoms, muramic acid, and ergosterol in samples of settled house dust. These substances serve as chemical markers of Gram-negative bacterial lipopolysaccharide, Gram-positive bacteria, bacterial peptidoglycan, and fungal biomass, respectively. A hydrolysate of 13C-labelled algal cells was used as an internal standard for first three markers. These analyses required two dust samples, one for 3-OH fatty acids, non-hydroxylated fatty acids and muramic acid, and another for ergosterol. The method can be useful for quantifying and characterising microbial communities in environmental samples.